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KMID : 0603820110170010039
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Journal of Experimental & Biomedical Science
2011 Volume.17 No. 1 p.39 ~ p.45
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Alteration of Inflammatory Cytokines by Volatile Organic Compounds in T Lymphocytes
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Lee Ji-Sook
Kim In-Sik
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Abstract
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In the present study, we investigated whether volatile organic compounds induce inflammatory response in human T lymphocytes by evaluating the alteration of inflammatory cytokines. Volatile organic compounds such as formaldehyde, o-xylene, benzene, and hydroquinone have no cytotoxic effects on Jurkat T cells at a high concentration of 50 μM for 48 h. IL-2, IL-4, IL-13, TNF-α and IFN-γ were increased after the treatment with volatile organic compounds, although alteration of cytokines is different among volatile organic compounds. LPS as a positive control increased the secretion of IL-2, IL-4, IL-13, TNF-α and IFN-γ. MCP-1 and CCL17 (thymus and activation-regulated chemokine, TARC) were weakly increased after the treatment with volatile organic compounds but the amount of the increased cytokine was below 20 pg/ml. These results suggest that the measurement of cytokine in Jurkat T cells may be used as a useful method for evaluating the toxicity of volatile organic compounds in immune response.
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KEYWORD
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Volatile organic compounds, Inflammatory cytokine, T cells
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